ELISA analysis in R

OK, here’s a little taste of what I usually do, rather than some random ramblings… I have a PhD student who is running enzyme-linked immunosorbent assays, or “ELISAs” to the initiated. This is a standard technique for measuring (in a semi-quantitative way) the amount of a given substance in a sample. Basically (and this is a pretty basic description), you stick an antigen to a surface (like the bottom of a plastic tray) and then add an antibody that sticks to the antigen. The antibody has an enzyme stuck to it and when you add the substance of interest the enzyme catalyses a reaction that causes a change in the intensity of a colour. You can shine a light through the sample to see how much the colour has changed and that gives you an idea about the concentration of your sample.Read More »

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Leeds Big Data Week (big data for conservation biology)

I’m excited to be a part of Big Data Week this year. For those of you who aren’t familiar with the phenomenon of big data, IBM has a pretty good definition.  In essence, we are collecting huge amounts of data by virtue of living in a technologically advanced world, and those data are collected rapidly in a diverse range of formats. The challenge now is what to do with all of it! Big Data Week, which is running from 22-28 April 2013, is an international movement that was established in 2011 to connect businesses, data scientists, and technology groups to explore novel social, political, technological and commercial applications of big data.  Leeds Data Thing is my local big data group, formed in 2013 to provide a venue for the discussion of local big data applications.  They are putting on a range of events for BDW 2013, and I have volunteered to give a short presentation at one of those events.

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