ELISA analysis in R

OK, here’s a little taste of what I usually do, rather than some random ramblings… I have a PhD student who is running enzyme-linked immunosorbent assays, or “ELISAs” to the initiated. This is a standard technique for measuring (in a semi-quantitative way) the amount of a given substance in a sample. Basically (and this is a pretty basic description), you stick an antigen to a surface (like the bottom of a plastic tray) and then add an antibody that sticks to the antigen. The antibody has an enzyme stuck to it and when you add the substance of interest the enzyme catalyses a reaction that causes a change in the intensity of a colour. You can shine a light through the sample to see how much the colour has changed and that gives you an idea about the concentration of your sample.Read More »

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iPad apps for academics (Part 2)

I wrote earlier about a few apps that I had found useful in my first weeks of owning an iPad. Well I’ve been actively pursuing opportunities to learn more about the learning applications for tablets like the iPad and wanted to share some of what I have found. A lot of this comes from a workshop by the brilliant Joe Moretti, who came to my university to run a workshop on iPads in education. I hope these are useful to you, too:Read More »